A26 - Modelling mechanisms of membrane shaping, fusion, and fission in endo- and exocytosis by protein scaffolds

Principal Investigator

Prof. Dr. Michael Kozlov, Tel Aviv University, Israel

We will use the theory of membrane elasticity to address key mechanisms of membrane remodelling in: clathrin-mediated endocytosis mediated by BAR family proteins in conjunction with dynamin and clathrin adaptors; clathrin independent endocytosis at synapses mediated by actin-myosin complexes; and synaptic vesicle exocytosis in the presynaptic active zone mediated by scaffolding proteins. The goal is to provide testable predictions for the experimental projects (A01), (A07), (A12), (Z02), (A03), (A05), and (A06) and complement the results of numerical simulations performed by (A04).


  • Choudhary, V., G. Golani, A.S. Joshi, S. Cottier, R. Schneiter, W.A. Prinz, and M.M. Kozlov. 2018. Architecture of Lipid Droplets in Endoplasmic Reticulum Is Determined by Phospholipid Intrinsic Curvature. Curr Biol. 28(6),915-926.
  • Wu, X.S., S. Elias, H. Liu, J. Heureaux, P.J. Wen, A.P. Liu, M.M. Kozlov, and L.G. Wu. 2017. Membrane Tension Inhibits Rapid and Slow Endocytosis in Secretory Cells. Biophys. J. 113 (11), 2406-2414.
  • Schweitzer, Y., and M.M. Kozlov. 2015. Membrane-Mediated Interaction between Strongly Anisotropic Protein Scaffolds. PLoS Comp. Biol. 11(2): 2 e1004054.
  • Schweitzer, Y., T. Shemesh, and M.M. Kozlov. 2015. A Model for Shaping Membrane Sheets by Protein Scaffolds. Biophys. J. 109(3): 564-573.
  • Terasaki, M., T. Shemesh, N. Kasthuri, R.W. Klemm, R. Schalek, K.J. Hayworth, A.R. Hand, M. Yankova, G. Huber, J.W. Lichtman, T.A. Rapoport, and M.M. Kozlov. 2013. Stacked endoplasmic reticulum sheets are connected by helicoidal membrane motifs. Cell. 154:285-96.

  • Elia, N., G. Fabrikant, M.M. Kozlov, J. Lippincott-Schwartz. 2012. Computational model for cytokinetic abscission driven by ESCRT-III polymerization and remodeling. Biophys. J. 102: 2309-2320.

  • Boucrot, E., A. Pick, G. Camdere, N. Liska, E. Evergren, H.T. McMahon, and M.M. Kozlov. 2012. Hydrophobic insertions promote, while crescent BAR scaffolds limit vesicle membrane fission. Cell. 149: 124-136.

  • Shibata, Y., T. Shemesh, W.A. Prinz, A.F. Palazzo, M.M. Kozlov, and T.A. Rapoport. 2010. Mechanisms determining the morphology of the peripheral ER. Cell. 143:774-88.

  • McMahon, H.T., M.M. Kozlov, and S. Martens. 2010. Membrane curvature in synaptic vesicle fusion and beyond. Cell. 140:601-605.

  • Campelo, F., G. Fabrikant, H.T. McMahon, and M.M. Kozlov. 2010. Modeling membrane shaping by proteins: focus on EHD2 and N-BAR domains. FEBS Lett. 584:1830-9.