A01 - Structural and functional organization fo endocytic scaffolds within the periactive zone
Communication within the nervous system involves the exocytic fusion of neurotransmitter-filled synaptic vesicles (SVs), neurosecretory organelles that comprise distinct sets of proteins and lipids, at active zone membranes. Following exocytic fusion SVs are recycled locally (1) (Figure 1) largely via clathrin-mediated endocytosis (CME) of SV proteins within the surrounding periactive zone. Clathrin-based SV recycling in addition to clathrin (2) involves endocytic adaptors such as stonin 2 (3) and AP180 (4) as well as accessory proteins that contribute to SV cargo sorting, membrane deformation, remodelling, and fission. Although substantial progress has been made towards unravelling the mechanics of CME, the spatio-temporal dynamics of endocytic proteins within the endocytic or periactive zone and the underlying molecular mechanisms have remained elusive. Such spatio-temporal control of the SV cycle within the nerve terminal likely involves membrane-associated multidomain scaffolds including intersectin (5) (Figure 2) and Eps15.
Within project A01 we investigate the role of Eps15/intersectin as a molecular scaffold linking SV sorting adaptors such as stonin 2/stoned B to periactive zone organization. We make combined use of cell biological, biochemical, and genetic approaches, paired with electron and super-resolution light microscopy (SRLM) techniques to unravel the structural and functional organization of the Eps15/ intersectin-based endocytic scaffold at synapses. On the long run we expect to gain new insights into the role of endocytic scaffolds in presynaptic organization and exo-endocytic coupling.
Fig. 1: Schematic depiction of the exo-endocytic cycle of SVs. The AZ is a specialized matrix for exocytosis, whereas SV endocytosis preferentially occurs at the surrounding periactive zone. SV proteins other than synaptobrevin are summarized as "cargo proteins" (taken from Haucke et al., Nat Rev Neurosci 2011).
Fig. 2: Domain structure and protein interactions of intersectin 1L (ITSN1-L) (taken from Pechstein et al., Biochem. Soc. Trans. 2010).
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Koo, S.Y., Markovic, S., Puchkov, D., Mahrenholz, C., Beceren-Braun, F., Maritzen, T., Dernedde, J., Volkmer, R., Oschkinat, O., Haucke, V. (2011) SNARE motif-mediated sorting of synaptobrevin by the endocytic adaptors CALM and AP180 at synapses. Proc. Natl. Acad. Sci. USA, 108, [advance online publication]
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