Z05 - Three-dimensional cryo-electron microscopy

Prinzipal Investigator

Prof. Dr. Christian Spahn, Charité Berlin

The overall aim of project Z05 is to facilitate 3D cryo-electron microscopy (3D cryo-EM) analyses of membrane scaffolding. In close collaboration with the projects of the SFB958, they will foster molecular cryo-EM to determine the structure of isolated macromolecular assemblies by the single particle approach or by molecular cryo-electron tomography (cryo-ET) in combination with sub-tomogram averaging. Furthermore, they will newly implement the workflow for cellular cryo-ET for visualizing membrane scaffolds in the native environment of the cell.

References:

  • Qureshi, B.M., A. Schmidt, E. Behrmann, J. Bürger, T. Mielke, C.M.T. Spahn, M. Heck, and P. Scheerer. 2018. Mechanistic insights into the role of prenyl-binding protein PrBP/δ in membrane dissociation of phosphodiesterase 6. Nat Commun. 9:90.
  • Said, N., F. Krupp, E. Anedchenko, K.F. Santos, O. Dybkov, Y.H. Huang, C.T. Lee, B. Loll, E. Behrmann, J. Bürger, T. Mielke, J. Loerke, H. Urlaub, C.M.T. Spahn, G. Weber, and M.C. Wahl. 2017. Structural basis for λN-dependent processive transcription antitermination. Nat Microbiol. 28;2:17062.
  • Hilal, T., H. Yamamoto, J. Loerke, J. Bürger, T. Mielke, and C.M. Spahn. 2016. Structural insights into ribosomal rescue by Dom34 and Hbs1 at near-atomic resolution. Nat Commun. 20;7:13521.
  • Sprink, T., D.J. Ramrath, H. Yamamoto, K. Yamamoto, J. Loerke, J. Ismer, P.W. Hildebrand, P. Scheerer, J. Bürger, T. Mielke, and C.M. Spahn. 2016. Structures of ribosome-bound initiation factor 2 reveal the mechanism of subunit association. Science Adv. 2(3): e1501502.
  • Behrmann, E., J. Loerke, T.V. Budkevich, K. Yamamoto, A. Schmidt, P.A. Penczek, M.R. Vos, J. Bürger, T. Mielke, P. Scheerer, and C.M.T. Spahn. 2015. Structural snapshots of actively translating human ribosomes. Cell. 161: 845 – 857.
  • Shah, C., B.G. Hegde, B. Morén, E. Behrmann, T. Mielke, G. Moenke, C.M.T. Spahn, R. Lundmark, O. Daumke, and R. Langen. 2014. Structural insights into membrane interaction and caveolar targeting of dynamin-like EHD2. Structure. 22: 409-20.
  • Fritsch, J., P. Scheerer, S. Frielingsdorf, S. Kroschinsky, B. Friedrich, O. Lenz, and C.M.T. Spahn. 2011. The crystal structure of an oxygen-tolerant hydrogenase uncovers a novel iron-sulphur centre. Nature. 479: 249-52.
  • Penczek, P.A., M. Kimmel, and C.M.T. Spahn. 2011. Identifying conformational states of macromolecules by eigen-analysis of resampled cryo-EM images. Structure. 19: 1528-90.