Z02 - Advanced and super-resolution imaging to resolve the nanoscale structure and dynamics of membrane scaffolds

Principal Investigator

Dr. Jan Schmoranzer, Charité

Prof. Dr. Helge Ewers, FU Berlin

TP Z02 develops, optimizes and applies advanced imaging technology (super-resolution, single particle tracking) to resolve the spatiotemporal organization of membrane scaffolds that are hidden behind the diffraction barrier. For the next funding period we will further apply and improve those methods aiming at analyzing the structure, dynamics and stoichiometries of single molecules within dense, nanoscopic membrane scaffolds.

References:

  • Gao, M., Maraspini, R., Beutel, O., Zehtabian, A., Eickholt, B., Honigmann, A.* and Ewers, H.* Expansion stimulated emission depletion Microscopy (ExSTED) ACS Nano 12, 4178-4185 (2018).

  • Schöneberg, J., M. Lehmann, A. Ullrich, Y. Posor, W.-T. Lo, G. Lichtner, J. Schmoranzer*, V. Haucke*, and F. Noé*. 2017. “Lipid-Mediated PX-BAR Domain Recruitment Couples Local Membrane Constriction to Endocytic Vesicle Fission.” Nature Comm. 8: 15873 (2017).
  • Albrecht, D., Winterflood, C. M., Sadeghi, M., Tschager, T., Noé, F., and Ewers, H. Nanoscopic compartmentalization of membrane protein motion at the axon initial segment. J Cell Biol. 215, 37–46 (2016).
  • Lehmann, M., G. Lichtner, H. Klenz, and J. Schmoranzer. Novel Organic Dyes for Multicolor Localization-Based Super-Resolution Microscopy. J. of Biophotonics 9 (1–2): 161–70 (2016).
  • Gimber, N., G. Tadeus, T. Maritzen, J. Schmoranzer*, and V. Haucke*. Diffusional Spread and Confinement of Newly Exocytosed Synaptic Vesicle Proteins. Nature Comm. 6: 8392 (2015).
  • Lehmann, M., B. Gottschalk, D. Puchkov, P. Schmieder, S. Schwagerus, C.P.R. Hackenberger, V. Haucke, and J. Schmoranzer. Multicolor Caged DSTORM Resolves the Ultrastructure of Synaptic Vesicles in the Brain. Angew. Ch. Int. Ed. 54 (45): 13230–35 (2015).
  • Mikhaylova M., Cloin, B.M.C., Finan, K., van den Berg, R., Teeuw, J., Kijanka, M.M., Sokolowski, M., Katrukha, E.A., Maidorn, M. Opazo, F., Moutel, S., Vantard, M. Perez, F.,van Bergen en Henegouwen, P.M.P., Hoogenraad, C.C., Ewers, H.*, and Kapitein, L.C.* Resolving bundled microtubules using anti-tubulin nanobodies. Nat Comm 11; 6:7933 (2015).
  • Kaplan, C., Jing, B., Winterflood, C.W., Bridges, A.A., Occhipinti, P., Schmied, J., Grinhagens, S., Gronemeyer, T., Tinnefeld, P., Gladfelter, A.S., Ries, J. and Ewers, H. The absolute arrangement of subunits in cytoskeletal septin filaments in cells measured by fluorescence microscopy. Nano Letters 15(6):3859-64 (2015).
  • Winterflood, C.M., Platonova, E., Albrecht, D. and Ewers H. Simple and robust dual color 3D superresolution microscopy by combined spectral-demixing and bi-plane imaging. Biophysical Journal 109 (1) pp:3-6 (2015).
  • Sakaba T., Kononenko N., Pechstein A., *Schmoranzer J*., Yao L., Barth H., Aktories K., Haucke V., “Exo-endocytotic coupling mediated by intersectin at a central synapse”, * in press, PNAS*   
  • Podufall J., Tian R., Knoche E., Jung N., Lampe A., Wichmann C., Zhang Y.Q., *Schmoranzer J*., Sigrist S.J.,  Haucke V., “A presynaptic role for the cytomatrix protein GIT in synaptic vesicle recycling”, *manuscript in preparation*    16. Hucho T., Suckow V., Joseph E.K., Kuhn J., *Schmoranzer J*., Dina O.A., Chen X., Karst M., Bernateck M., Levine J.D., Ropers H.-H, "Ca++/CaMKII switches nociceptor sensitizing stimuli into desensitizing stimuli", *J. Neurochemistry,** *2012 Nov;123(4):589-601*.*   
  • Lampe A.,* *Haucke V., Sigrist S., Heilemann M., *Schmoranzer J*, ”* *Multi-color direct STORM with red-emitting carbocyanines”, *Biology of the Cell*, 2012 Apr;104(4):229-37.   
  • Schmoranzer J*, Fawcett JP, Segura M, Vallee RB, Pawson T, Gundersen GG, ”Par3 and dynein associate to regulate local microtubule dynamics and centrosome orientation during migration”, *Current Biology,*2009;19(13):1065-74.   
  • Bartolini F, Moseley JB, *Schmoranzer J*, Cassimeris L, Goode BL, Gundersen GG, “The formin mDia2 stabilizes microtubules independently of its actin nucleation activity”, *J. Cell Biology*, 2008;181(3):523-36* *    
  • Gundersen GG, Wen Y, Eng CH, *Schmoranzer J*, Canbera-Poch N, Morris EJS, Chen M, Gomes E,  “Regulation of microtubules by Rho GTPases in migrating cells”, *Novartis Found Symp*. 2005;269:106-16  * * 
  • Wen Y, Eng CH, *Schmoranzer J*, Canbera-Poch N, Morris EJS, Chen M, Wallar BJ, Alberts AS, Gundersen GG “EB1 and APC interact with mDia to selectively stabilize microtubules downstream of Rho and promote cell migration”, *Nature Cell Biology*, 2004;6(9):820-30  * * 
  • Schmoranzer J*, Kreitzer G and Simon SM “Migrating fibroblasts perform polarized and micro-tubule-dependent exocytosis towards the leading edge”, *J. Cell Science*, 2003;116(22):4513-19   
  • Schmoranzer J *and Simon SM “Role of microtubules in transport and fusion of post-Golgi vesicles at the plasma membrane” *Mol. Biol. Cell*, 2003;14(4):1558-69   
  • Kreitzer G, *Schmoranzer J*, Gan Y, Low SH, Zhang Z, Weimbs T, Simon SM and Rodriguez-Boulan E* *“Three dimensional analysis of post-Golgi carrier exocytosis in polarized epithelial cells”, *Nature Cell Biology*, 5(2):126-36, Feb 5, 2003 
  • Lampson MA, Schmoranzer J, Zeigerer A, Simon SM, and McGraw TE “Insulin-regulated release from the endosomal recycling compartment is regulated by budding of specialized vesicles”, *Mol. Biol. Cell*, 2001;12(11):3489-501