A25 - Nanoscale organization of adaptor scaffolds involved in polarized cargo sorting at the trans-Golgi network (TGN)
In project A25 we will investigate the sub-compartmental nanodomain organization of the trans-Golgi network (TGN) that likely underlies the spatio-temporally controlled sorting of distinct cargos to different organelles or plasma membrane domains. Harnessing CRISPR/Cas9 knock-in cell lines for live-cell super-resolution imaging and acute protein degradation strategies, we aim to dissect the functional role of adaptor-based membrane scaffolding proteins in TGN nanodomain organization to segregate and sort distinct membrane cargoes in polarized epithelial cells.
- Bottanelli, F., N. Kilian, A.M. Ernst, F. Rivera-Molina, L.K. Schroeder, E.B. Kromann, M.D. Lessard, R.S. Erdmann, A. Schepartz, D. Baddeley, J. Bewersdorf, D. Toomre, and J.E. Rothman. 2017. A novel physiological role for ARF1 in the formation of bi-directional tubules from the Golgi. Mol Biol Cell.
- Bottanelli, F., E.B. Kromann, E.S. Allgeyer, R.S. Erdmann, S. Wood Baguley, G. Sirinakis, A. Schepartz, D. Baddeley, D.K. Toomre, J.E. Rothman, and J. Bewersdorf. 2016. Two-colour live-cell nanoscale imaging of intracellular targets. Nat Commun. 7:10778.