Synapses exocytose synaptic vesicles (SVs) at specialized membrane sites called active zones (AZs) covered by protein scaffolds, which control SV exocytosis. Bruchpilot (BRP) is a major scaffold building block in Drosophila. Our recent work suggests that DSyd-1 orchestrates the assembly of the BRP-scaffold via trans-synaptic DNrx-DNlg1 signaling. Here we combine Drosophila genetics with in vivo imaging and super-resolution light and electron microscopy to address the role of DSyd-1 and DNrx/DNlg1 in BRP-scaffold assembly. Novel factors involved in DSyd-1- and DNrx/DNlg1-mediated BRP-scaffold assembly shall be identified and the dynamics underlying BRP-scaffold assembly and turnover will be investigated.
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